You are viewing a single comment's thread from:
RE: Lab Diaries #2 - Making My Own Transgenic Cell Line
I had to make a model system for my honors project, years ago! Unfortunately of my alloted 12 months for the project, the yeast wouldn't take their damn genes for 6 of them :/
So sorry to hear that! How did you make yeast cells to take the genes at the end? For human cancer cells, I added polybrene into the growth media, it should increase the efficiency of transduction... I actually managed to make my transgenic cells after the first try, maybe it was beginner's luck :)
In the end, my labmate got it down, I can't remember the specifics it was so long ago. We had an endless array of reasons it never worked... mistakes, contaminations, mistakes, bad lucks... mistakes.
It's awesome when things work the first time around! Well done :)
ha ha imagine if you working with mice instead of yeast. It seems easy on paper put in practice there are so many things that can go wrong.
Yeah, I can imagine that'd be a nightmare!