ANA Profile by Immunoblot

in #lakshmi2 months ago

An ANA Profile by Immunoblot is a type of blood test used to diagnose autoimmune diseases. It's a more specific follow-up test done after a positive ANA test using Indirect Immunofluorescence (IIF).

Blood Draw: A healthcare professional will draw a blood sample from your arm.
Immunoblotting: The blood serum is separated and exposed to strips containing various purified nuclear and cytoplasmic antigens.
Antibody Detection: If specific autoantibodies are present in your blood, they will bind to their corresponding antigens on the strips.
Analysis: Secondary antibodies linked to a detectable marker (often an enzyme) are used to identify the bound autoantibodies. This analysis reveals the specific antigen-antibody complexes present, indicating the type of autoantibodies.
Interpretation:

A positive ANA immunoblot result for a specific antibody helps diagnose a particular autoimmune disease. However, a negative result doesn't necessarily rule out an autoimmune condition. Your doctor will interpret the results in conjunction with your medical history, symptoms, and other laboratory tests.

If you want in more details please go for the below link.

https://hcmsolution.blogspot.com/2024/05/blood-tests-for-autoimmune-disorders.html

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