1H NMR Analysis of Non-Psychoactive Oxidized Hydrophilic Phyto-Cannabinoid - CB2 Agonist Concentrate (Proposed Treatment for Leukemia)

The following composition will express the interpretation of data received from 1H NMR spectroscopy analysis performed at the University of Ottawa facilities. The man operating the equipment is especially knowledgeable and an efficient person. Working alongside Glen Facey at the University was a remarkable occasion. He obtained the analysis results though we had no frame of reference for comparison.

Fortunately I have been writing about the subject for four years and knew where in the WWW to find the necessary reference data which allowed the interpretation of the analysis produced by 1H NMR spectroscopy. The data from previous scientific confirmations are coinciding with the expected results. Confirmation is necessary when composing solutions dealing with the preservation of life.

I am creating new samples with the aim of achieving a purity which will allow for a more detailed analysis of single compounds within the concentrate. The sample of concentrate analyzed below is a mixture of phyto-cannabinoids, the peaks of the most abundant molecular structures offer confirmation for certain aspects of the oxidized phyto-cannabinoid concentrate.

Figure. 1

Perspectives worthy of note:

The quantifiably greater electro-negativity of compounds in the concentrate demonstrate their polarity. It is interesting that all substances came from a water soluble fraction of extract. A discernable delimiting line (hydrophobic/hydrophilic) at the 5 ppm mark of the scale next to the 2H (deuterium) solvent peak at 4.8 ppm.

Figure. 2

Comparing with figure. 2, where THC graphs peaks in the 6 to 7 ppm range. Lower electro-negativity quantifies and confirms the compound's chemical property of being water insoluble.

Knowing that the concentrate contains only non-psychoactive phyto-cannabinoids (hydrophilic, CB2) is of great importance when considering the treatment of children. Those who have developed bone marrow cancers due to deficiency of endogenous cannabinoids specifically localized in bone marrow. Cells in bone marrow are characterized by their abundant cannabinoid receptor type two sites which have the chemical property of being hydrophilic and capable of interaction with hydrophilic compounds.

Endogenous cannabinoid 2-Arachidonoylglycerol and water soluble phyto-cannabinoids such as cannabinol, both are scientifically confirmed to cause apoptosis (cell death) of cancer cells. These water soluble compounds are effective when the biochemistry of the patient has them available in quantities sufficient to spare the resources and energy required in the production of endogenous cannabinoids.

Figure. 3

The peak with the largest area at 3.8 ppm on the x axis that designates the electronegativity of compounds, can be interpreted by referring to the table in figure. 3. The table lists the abundance of specific chemical structures which a molecule is composed of. In this case the peak at 3.8 corresponds to the standard signature of C-O (carbon-oxygen) bonds with CH3. This confirms an abundance of oxidized chemical compounds, while confirming a molecular structure which does not belong to the hydrophobic THC (6 - 7 ppm) nor does the peak correspond to hydrophilic CBN (0.9 - 2.4 ppm).

This confirms the presence of oxidized molecules without matches to existing data which was available. I need to ask if others have matches for oxidized phyto-cannabinoids recorded at 3.8 ppm. If no matches exist, the next step is to calculate the chemical structure of the compound. The compound is expected to be the result of an electrochemical process which is either the result of cannabinol's degradation or it is a novel compound being produced by electro-chemical process.

The following demonstrate the biochemical system by which the proposed treatment can be applied to causing apoptosis (cell death) of cancer cells. While sparing resources necessary in other biochemical processes of physical recovery.

Scientific confirmation which demonstrates the functions of cannabinoids and cannabinoid receptors in marrow cells and bone structure.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2219540/

This document demonstrates the work of others in finding hydrophilic ligands which can be used as cannabinoid receptor type two agonists. This work also corrects the diagram and findings of the earlier work cited above (2008, Cannabinoid receptors and the regulation of bone mass).
https://www.frontiersin.org/articles/10.3389/fphar.2018.01202/full

After having considered the information above, the problem with the following diagram should become apparent. Can you find the necessary correction?

..hint, today we know that CB1 receptors are themselves hydrophobic and CB2 receptors hydrophilic. Anadamide is a hydrophobic compound due to it's low electro-negativity (non-polar). 2-Arachidonoylglycerol is a hydrophilic compound due to it's high electro-negativity (polar). THC is hydrophobic along with it's chemically associated receptor CB1. CBN is hydrophilic along with it's receptor CB2.

In case it has yet to become apparent, substitute the triangles in the diagram for squares and squares for triangles. Suddenly the interaction between cannabinoids and their receptors becomes logical while respecting principles of chemistry. Such as electro-negativity, a quantifiable measure of a compound being hydrophilic or hydrophobic.