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RE: Determining the Gene responsible for providing the antimicrobial property from the Bacterial Genome
Are you doing all the work from peptide purification to Mass Spectrometry to genome sequencing. If so, bravo!
Yeah, we isolate the peptide from bacterial source, purify them and do all the characterization needed for the peptide and checking their activity in the cancer cell lines also, some of the peptides show effective killing in cancer cells also.
One of the peptide from our lab shows killing of leishmania also.
And species whose 16SrRNA shows similarity % less than 96%, we go for whole genome. We are also trying to clone the peptide sequence and produce them in E.coli. This work is going on in Dr. Bob Hankock lab in University of British Columbia, Vancouver.