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RE: Targeted Protein Labeling Through Amber Codon Suppression Technology
Greetings @justtryme90 !Very interesting and promising this work, I understand that the mechanism of action is similar to that of tumor markers? and on the other hand, would the function of the protein be affected?
Excellent question!! The function of the protein can very much be affected, this is a possibility in any covalent modification of a protein, and even more so by the addition of a non natural amino acid. However one can do a variety of standard biochemical assays to probe enzyme function and if things do not appear significantly perturbed (greater than a 5% deviation from wild type rates) then we would likely suspect that the labeling is not causing a significant difference. However we would not actually know! That's one of the drawbacks to studying things this way. We have no better way to see these movements really, so it's a drawback that we have to mitigate as best we can!
You must continue with the investigation, suddenly you will find new answers and serendipities....
Well someone must at least :D