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RE: mRNA modifications to overcome stability and delivery issues

in #steemstem3 years ago (edited)

Try Mg2+ ions to neutralize negative charge of mRNA. Most nucleic acids exist in vivo as Mg2+ complexes, not just regular phosphate salts. However, the natural Mg2+ complexes may be more susceptible to RNAases! Use RNAase inhibitors, consider substituting Mn2+, or Zn2+, or Co2+, instead of Mg2+?

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Unfortunately, if you deliver mRNA with RNAse inhibitors the cells will be all messed up.

Next thing you know, you'll be telling me we can't inject people with guanidinium thiocyanate to prevent RNA degradation either! ;)