Hey, thanks for the response

do you mean yo-yo effect?

yes I mean this. I will edit it. Thank you.

This is some nice data, do you have a dose-response showing at what concentration of rapamycin the onset of phosphorylation inhibition occurs in cells? I am curious what the IC50 is.

The IC50 depends on the cells you are using. In HEK, PC-3, EA.hy I used (it's been a while since I did it) less than 20 nM for viability experiments and 50 to 100 nM for Western I think.

Perhaps it is really the extreme high-fat of the atkins diet that eventually catches up with you. Maybe fat shuttling processes get overwhelmed? Do people on atkins diets typically have super high VLDL levels? Have there been any studies that track VLDL over time for people on these diets? Maybe its the triglycerides that get ya?

I have no idea. I will research this. Hang on!

What is the optimal dose of 2-DG to achieve a response? If its too high then its likely truly not clinically relevant. Nobody wants to eat a burrito of something for a treatment. :D

Due to it's in competition with usual sugar you need a lot.

Feeding 2DG (0.4%) to Sprague-Dawley rats for 6 months, Wan et al.(2003) observed reduced serum glucose and insulin concentra-tions and increased ACTH and corticosterone levels, comparableto rats on a CR regimen.

Keep your fingers off vitamin supplementation

In any case! ;-)

Nah, its got really really bad bioavailability! There are some studies which indicate that its metabolites may also have an effect, but I wouldn't really put a lot of stock into it really having strong in vivo efficacy for much of anything.

That's definitely right. Nevertheless, there are some clinical data supporting the use of trans-resveratrol. Check my autophagy articles as well as Roth and Ingram 2014 for further information.

You did a lot of nice research here, really well done job!

Thank you very much. I appreaciate constructive feedback like yours!

Cheers

Chapper