Found this growing on a petri dish in the lab...

in #science6 years ago

Hello Steemit world,

I was cleaning up the lab for the holidays when I found a contaminated LB agar plate (poorly poured at that) growing an interesting looking lawn of bacteria on the surface. The media had 100ug/mL of chloramphenicol, so it should have been enough antibiotic to keep any unwanted critters off the dish — but for this one it didn't. This implied whatever was growing had some antibiotic resistance, which for some bacteria arises naturally or is innate.

"So much for that plate...also nice pouring job..."
20171219_152603_HDR.jpg

This was the only contaminated dish with this microbe of the 20 or so which were poured. The plate was kept closed with the lid in the biological safety cabinet at room temperature the entire time since pouring, so contamination must have arisen somehow from handling, or at the time of pouring.

At first glance I had just noticed the contaminated plate, but not the intricate colony morphology on it. Closer inspection revealed that these bacterial growths had distinct branch points and spiraling arms that spread from an initial central colony. These growths resembled snowflakes, or how frost forms branching patterns as water crystallizes; but there was also rotation within the arms and branch points.

"Wait, what is THAT?"
[crop large] Contaminated plate 20171219_153413_HDR.jpg

(The only camera that was at my disposal was on my phone, so I tried to take the best photos I could — please excuse the resolution and focus.)

Zooming in closer...
[crop small] Contaminated plate 20171219_153413_HDR.jpg

If I have correctly identified this microbe it is Paenibacillus dendritiformis, which has complex population dynamics and social behavior that governs the motility and branching as it grows. It is known to be multi-drug resistant, which explains the growth on LB agar with 100ug/mL of chloramphenicol. Interestingly, this critter employs such complex intercellular chemical communication that it can behave as a socially intelligent multicellular community, despite P. dendritiformis being a single-celled organism. [I know it's deemed lazy and sloppy to reference Wikipedia, but given it's the holidays I'm feeling pretty lazy; so, I'll reference it anyways because the primary literature is cited within and the page checks out: https://en.wikipedia.org/wiki/Paenibacillus_dendritiformis]

Given the complex colony architecture and morphology there are numerous false-colored images of this bacterium across the web. Some show the branching and adventurous growth in detail, and others are more psychedelic. If you Google image search the microbe name you will see what I mean. Here is an example:

"FOR THE HIVE!"
Critters.png

If you are familiar with microbiology I hope you have enjoyed this posting and found it informative. If you are not familiar with this subject I similarly hope it was enjoyable and has piqued your interest into an often unseen world that can govern health, disease, medicine, and of course beer and wine.

Constructive feedback is always welcome to improve the quality of content; after all I'm a scientist, not a writer!

Cheers!

Sort:  

This is intense. Diggin' it.

Glad you enjoyed it!

I like how you used a lab anecdote to share some cool information about this micro-organism, definitely made me want to learn more about it.

Cheers

Glad you liked it! :)

Cheers!

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Looks more like a dehydrated plate then an organism growing...

It may have been a bit dehydrated because the plate was old, but there were definitely microorganisms on it. Look at the morphology and compare it to just a dehydrated plate where the ridges and faults would be much larger. Also, I compared this dish directly to the same plates (LB+Cm @100ug/mL) that were vacant for growth; this one definitely had critters on it.

How could this be interpreted as anything other than a confluent lawn? Look at the first photograph where the agar is thin where there isn't growth and compare with the rest. It is evident there is a boundary for growth there the nutrients were minimal.

I'm not convinced. It's just hard for me to tell with these images. I've had plates that looked similar to this and they were just drying out. No microbial or fungal growth.

The patterns do indeed look neat, regardless.

Seen many dehydrated plates in my time, and this was certainly not one of them -- especially after I directly compared it to LB+Cm plates from the same batch that showed negligible rippling where they were beginning to dry out. Check out other photos of Paenibacillus to see what I'm talking about. Those branch points and patterns don't simply arise out of a dry plate; they would be much larger faults and not a separate hue/texture. This is definitely microbial growth on the surface. I can send you the original photos if you would like, but here is a couple enlarged images of the first photo:
[crop large] 20171219_152603_HDR.jpg

Aaannd another. I will draw your attention to the boundary where growth begins to stop. Why would the dehydrated plate not see more of those patterns where the nutrients/agar is minimal? I now turn the skepticism to your interpretation of the data (in true scientific fashion)
[crop small] 20171219_152603_HDR.jpg

Right click and go to "View image" to see the photos in more detail...

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