The alfalfa leafcutting bee

Megachile rotundata

finally i found an insects that did not fly away. i was able to shoot with different aperture. Here we can see the effects of Depth of Field.

Model: NIKON D7100
Exposure Time: 1/250 sec
Exposure Program: Manual
Exposure Bias: 0 EV
F Number: F 13
ISO Speed ratings: ISO 100
Flash: Flash fired [compulsory flash mode]
Focal Length: 60 mm
Metering Mode: Spot

F Number: F 13
ISO Speed ratings: ISO 100
Flash: Flash fired [compulsory flash mode]
Focal Length: 60 mm
Metering Mode: Spot

F Number: F 11
ISO Speed ratings: ISO 100
Flash: Flash fired [compulsory flash mode]
Focal Length: 60 mm
Metering Mode: Spot

F Number: F 9
ISO Speed ratings: ISO 100
Flash: Flash fired [compulsory flash mode]
Focal Length: 60 mm
Metering Mode: Spot

Model: NIKON D7100
Date/Time: 2019-09-21 09:09:42
Exposure Time: 1/250 sec
Exposure Program: Manual
Exposure Bias: 0 EV
F Number: F 8
ISO Speed ratings: ISO 100
Flash: Flash fired [compulsory flash mode]
Focal Length: 60 mm
Metering Mode: Spot

I am still finding the sweet aperture spot on my 60mm f2.8D macro lens. it really depends but for this 10mm insects i think f13 is best. The size of the insects and the distance from the subjects affects what aperture to used. btw, its a single shot.
Its not easy to do stacking shot in the field with a live insects :)