POLYMERASE CHAIN REACTION

Polymerase Chain Reaction developed by Kary Mullis in 1980s, was one of the greatest invention of the past century.....
It targets a segment of a gene and amplifies into millions of copies, it is also time and temperature dependent.
Steps involves

• Denaturation of the double stranded DNA at 94 Degree Celsius
  -Annealing of the primer to template at 55 Degree Celsius
  -Extension of the target sequence at 72 Degree Celsius
  PCR reagents involve
  -Primers
  -Buffers
  -Taq polymerase
  -Forward and Reverse primers
  -PCR grade water