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RE: My shittiest method: The in vivo complex of enzyme (ICE) assay.
seems you've got iron hands of analytical balance precision -- a stable pipette jockey!
there is really not much choice... you repeat the assay as long as you develop iron hands (that's probably a selection criteria for PhDs in our field and seem to carry the necessary gene). But your hands sure hurt afterwards!
looking forward to your shittiest method!