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RE: Cutting, Pasting, Cloning, and GMOs
Nice write up but most modern labs rarely use Restriction enzyme digestion for cloning anymore. Restriction enzyme independent cloning such as Gibson Assembly is a more efficient way and does not require restriction sites.
Yup. The post (like the series in general) is more of a historical affair, going over important discoveries in biology. It's certainly not cutting-edge or 'this just out' material.
Hey thanks for the upvote!
Cheers!
👍
I use Gibson assembly when I generate most plasmids, but sometimes you can't. But you'd be surprised how few people use Gibson assembly or even understand it. It's very useful and simple, so you'd think nearly everyone generating plasmids would use it...