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RE: Gene Editing - Cre/loxp (My Research, Vol. 2)

in #science7 years ago

Gene editing is a bit anti-climactic and is mostly imagination and your own knowledge.

Example: Mix a drop of this colorless liquid with a drop of that colorless liquid. Put that colorless liquid into cells. Wait a week or so for the cells to divide so that you have more of them, then check if the cells got the desired genetic modifications. So it is important that you have the knowledge of what components are inside each of the previously mentioned colorless liquids and what they are doing, but otherwise it looks the same to the observer.

I know this because I do it almost every day in human pluripotent stem cells.

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