How to Make Lactobacillus Serum

This stuff is expensive but you can just make your own.

Rice Wash

Get a container, fill halfway with rice-wash. Rice wash is the water leftover when you rinse fresh rice. For example, go buy rice, whatever kind, bring it home, put it in a pot with warm water, swirl it a bit and then drain the [now milky colored] water. The water is now a rich source of carbohydrates. In this step, you can substitute rice with another carbohydrate source if you don’t have rice, as long as it is complex (don’t use simple carbohydrates like sugar, honey, syrup, molasses, etc). You can use wheat, barley, kinoa, other carbohydrates as the base to make your carbohydrate wash. This wash will attract microbes from the air, among them lacto bacilli.

Cover loosely and let stand for a couple days to a week

When is it done?

When you see a light film on top (molds) and it smells a little sour and forms 3 layers. This is indicating the rice wash is infected with various microbes. This happens more quickly in warm temperatures because microbes are more active. Thus it is all relative since we don’t do this in controlled laboratory conditions.

The layers are distinct

1. Top layer: floating carbohydrates leftover from fermentation and possibly molds
2. Middle layer: Lactic Acid and other bacteria (cheese buffs will recognize this as a makeshift “rennet”). We will use this layer.
3. Bottom layer: Starch, by-product of fermentation

Extract the middle layer using a siphon.

This layer contains the highest concentration of lactic acid bacteria and lowest concentration of the unneeded by-products

Get a new container, larger than the first. Take the extracted serum from the last step and mix it with 10 parts milk. By saturating with milk (lactose), we dissuade other microbes from proliferating, leaving L. bacilli. E.G. if you have 1 cup of the serum, mix it with 10 cups milk.

The best milk to use in raw natural natural milk. However, any milk will do, even powdered milk. The best is unpasteurized natural but just use what is available. We just want to saturate with lactose to promote L. bacilli bacteria.

You want to keep this stage anaerobic as much as possible. You can use something like rice bran, barley bran, wheat bran, etc sprinkled on top of the milk. I use a sealed container with a one-way valve.

Note: Beware of bubbling during this phase. It can lead to overflows if you fill your container up. It can go through the one-way valves so keep an eye on it and don’t do this step around nice things.

Curds

After about 1 week (temp dependent), you’ll see curds (made of carbohydrate, protein, and fat) on top of the milk. The water below will be yellow colored – this is whey, enriched with lactic acid bacteria from the fermentation of the milk.

Microbes like L. bacilli are more active in warmer temperatures. The curds you see are a byproduct of the fermentation process. Fermentation is generally associated with microbial processes under anaerobic(no oxygen) conditions. Now, L. bacilli is a facultative anaerobe, that is it can live and work with or without oxygen, but less competition in anaerobic conditions.

The water below(whey+lacto) is the good stuff. You want to extract this. You can either skim the curds off the top, pour through a strainer, or whatever other methods to accomplish that

Remember the curds, or byproduct of milk fermentation by L. bacilli, are great food. They are full of beneficial microbes like L. bacilli. Feed the curds to the soil, compost pile, plants, animals, humans – whoever wants them! They are full of good nutrients/microbes. No waste in natural farming.

To preserve at room temperature, add an equal part sugar/molasses to the serum. So, if you have 1L of serum, add 1kilo sugar or 1L molasses. Otherwise store in fridge to keep.

Example Recipe:

1 L rice wash
add 10L Milk
After rice wash and milk remove curds – around 1L
Left with 10L pure LAB (lactic acid bacteria)
add 10kg sugar or 10L molasses
= 20 L stabilized lactic acid bacteria serum

What to use it for and how to use it

Before using, first mix 1:20 with water. 1 part serum to 20 parts water. Then follow instructions below:

Odor Reducer:

Add mixture to animal’s water at 2tbsp/L. You can mix it more or less, there are no rules here, just how we typically do it.

Apply to places where there is odor build up. The harmless bacteria “eat” the odor causing germs and the smell is gone!
Indoors: reduces foul odors, including animals like cats, dogs, mice, other pets. Stinky shoes? Wet clothes from being outside? Gym clothes that haven’t made it to the wash yet? Smoker in the house? Kill these nasty smells!
Outside: use to control odor in pens – pigs, cows, chickens. In barns, around the yard, etc

Household use:

Clear clogged drains: dump mixture into drain to clear clogs. Exact amount depends on the clog, haha. A few tbsp to 1L works well. For semi-clogged drains (like kitchen sink draining progressively slower), use at night and allow at least the night for microbes to work.

Keep septic clear. Tired of having your septic system drained? Add lacto! Depending on size of your system, pour a few tbsp. to a few L into the toilet every few months.

Houseplants:

Mix 2-3tbsp per 1L water and use that to water them.

Animal Bedding:

Mix 2tbsp to 1L water. Mix with animal bedding to reduce smell and increase longevity. In natural pig farming we use at least 1 yard deep of bedding so there is plenty of space for microbes to work. Bedding consists of organic substrate like rice hulls, wood chips, sawdust, wood shavings, shredded corn cob, any other high cellulose, high lignin material. Natural pig farming is a future topic on this site. Spray until bedding is slightly damp but not wet. How much you spray really depends on your climate. If you are in a very dry climate you can spray a little more and mix in evenly. Wetter (more humid) climates use a bit less. Mix into the bedding evenly where necessary (in many cases, like with pigs and chickens, they’ll mix it themselves). How much you use is all relative. These guidelines are for pigs and chickens. More extreme smells, just use more! Want to spray less often, use more! As we notice a smell we spray. Thus, as pigs grow bigger, make more poop, we spray more often! Dosage/frequency is relative and will depend on your situation.

Animals – Digestive/Growth Aid:

Mix 2tbsp to 1L water, then add that mixture to animal’s water at 2tbsp/L(so the animal’s water contains little less than a quarter tsp/L of lacto serum). But this is very flexible. The Lacto serum is not harmful, so its just about adding enough to be effective, without wasting it.

Improve digestive efficiency in humans and animals alike:

Improves how you feel after meals, particularly meals rich in meats. It’s awesome. After eating, mix 1-2tbsp lacto with a cup of water and drink that. Makes you feel so much better after! Lessens that afternoon lull, gives you more energy!

Aids digestion in animals.

This is critical. You can raise animals on less food, and see the same and greater growth rates. Amazing results in pigs . The principal is that the micro-organisms help digest the food coming in – better digestibility means better nutrient absorption. Save on feeds, better feed to growth conversion ratio!

If you really want to boost growth, mix 2tbsp to 1L water and soak the food in this solution for a few hours to a few days. Food is pre-digested when animals eat it, AWESOME!

Great results in livestock and poultry.

Plants – Growth Aid:

When added to water for plants, nutrient uptake efficiency is increased, which increases growth!

Improves growth of plants when applied as foliar spray and soil drench. Improves their efficiency in up taking nutrients so naturally, growth is enhanced. With the use of these micro-organisms, the nutrients you spray or drench to feed your plants become more bio-available and easily absorbable by the plants. Technically, you can say that plants do not use organic nutrients directly. Micro-organisms convert organic nutrients to their inorganic constituents which the plants utilize. Utilizing microbes, you will notice better plant growth and health.

Disease Resistance:

This is a consequence of the increased efficiency of nutrients. More nutrients available at smaller metabolic cost.
Lacto suppresses harmful bacteria in food/water that animals consume, enhances their gut flora so that line of defense
is working optimally, etc.

Aid Compost:

Mix 2tbsp/L and spray on compost pile to improve decomposition. This is a huge topic that will be expanded upon in another post.

Aid Organic Fertilizer:
Add 1-2tbsp per gallon water-nutrient solution. Lacto consumes organic nutrients making them bio-available to plant roots.

Plants don’t use organic fertilizer! Microbes break it down to inorganic constituents, and plants take those up. This product makes that process more efficient.

Aquaculture:

Lacto works in aquaculture just fine if you don’t have BIM available. Add lacto at roughly 1L per 700m3 of fish-containing water. Example: you have a pond that averages 20m wide by 30m long by 2m deep. So, 20 x 30 x 2 = 1200m3. In this case you would add roughly 1L of BIM or Lacto

Microbes digest fish wastes, cleaning up water and improving water quality.
Allows fish to grow larger due to digestive efficiency
Allows higher population of fish in the same amount of water! Literally, increases the carrying capacity of your body of water! This is awesome for aquaculture set ups

Disclaimer

I had this saved but the website is no longer active so I looked on the wayback machine and got it there.
I am posting this here to save it on the blockchain.