HOW-TO Make and Use Silver Thiosulfate Solution(STS) for Feminized Cannabis Pollen! (Featuring the Gorgeous Early Dane!)

in #cannabis6 years ago (edited)

Want Your Female Cannabis Plant Exploding With "FEMINIZED" Pollen Like This One?! This is HOWTO Feminize Cannabis!

Read on and see the simple steps required to produce drifts of FEMINIZED cannabis pollen!
Early Dane Spitting Feminized Pollen
Look at the flower clusters on her, they dumped an amazing amount of pollen for such a small plant.

Silver ThioSulfate Solution (STS) is Easy to Make and Apply!

The instructions I've provided here cover mixing, diluting, spraying tips and scheduling, along with additional tips from the pros. Even the most stubborn strain will reliably respond with feminized pollen when you carefully follow this tutorial. The materials you'll need are readily available nearly anywhere.

The ingredients you'll need are available on Amazon, the instructions are straightforward for mixing and the application is quick, repeatable and simple. It's taken me quite a while to make sure this is complete, I hope it helps you as much as it has me. :D

What About Hermaphrodites? (Hermies!!)

Here's what I've been able to find out about STS and hermies. STS does not promote hermies and it does not eliminate hermies. Creating a stress free flowering environment for your reversed females is VERY important. Here's why.

Reversing a female with STS will produce stable feminized pollen. At least as stable as the parents are anyway. Reversing a female known to hermie with light leaks, in a room with a light leak, will create both feminized & hermie pollen. You will get female and hermie seeds. The same is true for any plant with hermie triggers that is flowered in a space which has those triggers.

The difference being, you'll be unable to tell which flowers are producing hermie pollen and which are producing valuable feminized pollen. The flowers look the same. So it's extremely important to reverse females in a space you know will not trigger hermie tendencies. It would be wonderful to work with genetics without hermie traits. I look forward to the day when this will be a commonplace event. ;) Until then we make a great many seeds, so we can stress test females and find the ones which do not hermie. :)

ITEMS YOU WILL NEED WHEN MAKING STS:

Mixing the A and B "Stock" Solutions

STS is stored as two solutions, A & B. When kept sealed, cool and without exposure to light, they are very stable. Separately, they can be stored for months without issue. Once mixed their life span is about 1 month, but only if kept cool, sealed and in the dark. ;) This mix is diluted with r/o water before spraying on plants.

Bottles, Sprayer and Chems

Stock Solution A

  • Fill the pyrex 2-cup measure to the 500ml line with r/o water
  • Measure .5 Grams of Silver Nitrate using the digital scale
  • Slowly pour the silver nitrate crystals in to the 500ml of r/o water while stirring
  • Stir until dissolved (15 seconds)
  • Pour the solution in to the 500ml brown glass bottle and cap it tightly
  • LABEL the bottle "STS PART A" in a clear and long lasting fashion
  • Thoroughly wash and rinse the pyrex measuring cup

Stock Solution B

  • Fill the pyrex 2-cup measure to the 500ml mark with r/o water
  • Measure 2.5 Grams of Sodium Thiosulfate Anhydrous OR 3.9Grams of Sodium Thiosulfate Pentahydrate
  • Add the Sodium Thiosulfate to the r/o water while stirring
  • Stir until completely dissolved (30-45 seconds)
  • Pour the Sodium Thiosulfate solution in to a 500ml brown glass bottle and cap it tightly
  • LABEL the bottle "STS PART B" in a clear and long lasting fashion
  • Throughly wash and rinse the pyrex measuring cup

I store these solutions in the 500ml brown glass bottles and mix fresh before diluting for spraying. It is best to keep them in the fridge. ;)

Mixing and Diluting the Stock Solutions Before Spraying

ALWAYS Mix Part A in to Part B! This means putting part B in a container first and then adding part A while stirring. The measurements listed here make 1 quart, which fits the size of the sprayer and bottles I posted above. This amount provides plenty of solution for a 2' tall plant, sprayed every 5 days for 30 days.

When stored in a dark cool place, the mixed solution is effective for approximately 1 month. The A and B solutions will be effective for at least 6 months.

For 1 Quart of Solution: which will fit the sprayer listed:

  • Use a 60cc syringe and suck up 50ml of Part B solution
  • Gently squirt the Part B solution in to the Pyrex measuring cup
  • Completely rinse the syringe or use a second clean syringe
  • Suck up 50cc of Part B Solution
  • While stirring the stock solution in the measuring cup rapidly (I use a small Stainless Steel Whisk), gently squirt the 50cc's of Part A solution in to the measuring cup. Go slowly, yet quickly enough to completely mix them within 30 seconds.

REMEMBER it's IMPORTANT to DILUTE!

Without dilution this solution will burn plants When the above directions are followed you will have 100ml of mixed stock solution in a pyrex measuring cup. Pour this solution into the 1 quart, brown glass bottle with the sprayer top and then fill the bottle the rest of the way with r/o or distilled water. (800+ml)

This dilution rate is very close to a 1:9, stock:water mixture. Add 6-10 drops of Dawn Ultra dish soap (or other soap/surfactant, some people use yucca extract), shake gently and spray until the soap mixture is being sprayed. Test it on a leaf. The mixture should stick to the leaf without beading up and rolling off.

If necessary, add additional drops of soap until the solution sticks/spreads well. You can also use Yucca extract as a spreader/surfactant, instead of the dish soap. (I cannot reccomend Tween-80 even though other have said it works. I personally cannot use it without having serious memory, mood and logic problems when exposed to the Polysorbate in Tween-80.)

Light Proof the Sprayer Bottle!

I know it's a brown glass bottle and a black plastic top, I still covered it further. Since I keep it in the fridge, there is definitely strong light when the door is open. ;) I put the sprayer in a black plastic trash bag and folded it in a few layers around the bottle, then taped it in place. It's not sexy, but it works. :) You can see it in the photo of the chemicals and bottles above.

Staying Safe!

WEAR PERSONAL PROTECTION GEAR, including gloves, mask and goggles/glasses. You'll want to keep this off your skin and out of your mouth/lungs. Sodium Thiosulfate has no listed toxicity but it is an irritant with repeated exposure. Silver nitrate will stain surfaces brown, only light stains when mixed with sodium thiosulfate and diluted.

I sprayed my hand once, it felt 'cold' for days. Not a good sign, so I'm careful to protect myself from spray and inhalation. The solution rinses off of gloves and surfaces just fine, change out of any clothing you accidentally spill it on.

Spraying Methods and Schedule

That being said, the process of treating your plants with STS is very straightforward. There's debate about spraying each node or using an eye-dropper on each node. I use the spray method and I know it works.

  • Spray Every 5 Days After Lights Out
    Silver Thiosulfate stops the production of ethylene. This effect only lasts a few days in cannabis and spraying every 5 days keeps ethylene production the lowest. Turn off the main lights and spray before the night cycle. Silver thiosulfate is sensitive to light, so low lighting and then dark works well.

  • Dilute Further if Burning/Yellowing Develops
    Some strains/phenotypes of cannabis are more sensitive than others. Should yellowing or burning develop, pour out some of the solution in the sprayer (with lots of rinse water down the drain) and add distilled or r/o water.

  • Keep STS Out of the Root Zone
    Cover the base area of the plant with newspapers before spraying. Make sure the plant has completely stopped dripping before removing the newspapers or other barriers.

  • Saturate Each Node With Spray
    The nodes are located at each spot where a leaf joins a branch. During vegetative growth, each node will eventually produce another branch. During flowering, each node produces flowers.

  • Turn Off Fans Before Pollen Begins Dropping
    Pollen is insidious and will float in to every crack and corner of your house. HEPA filtered ventilation (even HEPA rated furnace filters work) will significantly reduce the spread of pollen. Spray down all pollen covered areas with clean water to deactivate pollen.

  • Dilute Solution if Plants Show Burning
    A slight reaction is fine, while severe reactions require diluting with additional distilled or r/o water. Open up the spray bottle and top it off with about 1/3rd of a cup of distilled or r/o water. Pour a bit out first, if there won't be room.

What You Should Expect to See

You sprayed down your female, gave her 5 days more veg and sprayed her again. You changed the lighting to flowering and have sprayed her every 5 days for a couple weeks. Here's an example of what you could be looking at. In the photo below you can see the male flowers, the parts which look like balls or seed pods, and also the white pistils of the female flowers.
Early Dane Almost Spitting Pollen

Although I Prefer Parchment Paper for Collecting Pollen

you can see I used aluminum foil for this project. The foil was placed under the branches to collect dropping pollen. In the photo below, you can see the foil has a dusting of pollen on it, with small drifts building up at the creases.
Foil-Catching-Pollen-Dropping.png

Here You Can See a Foil Cupcake Cup

with pollen and male flowers in it from one piece of foil.
Collected-Pollen-ready-for-dessicating-small.png

Lastly: Does it Pollinate and Make Seeds?!?!

What do you think? This Early Dane feminized pollen has already been used to pollinate another Early Dane I had growing. You can see in the photo of her below, the pistils are all darkened and seeds are forming from definitely viable pollen! The seeds forming are going to be 99.9% female plants.
640-Early-Dane-S1-seeds-forming.png

Here She Is a Few Weeks Later

You can see the seeds have grown quite a bit and are now breaking open the seed bracts covering them. An amazing sight for me to see, after a decade and a half of avoiding them. ;)
Link to Full Size Image
Early-Dane-S1-Splitting-Open-1024.png

Some Tips From the Pros

I've searched the internet for as much information as I could find on using STS and working with pollen. I found a few pieces of information which some of you may find helpful. The first two are mine.

  • Trim Everything But The Top Three Nodes of Each Branch
    You want the plant to focus a lot of energy on perfectly formed flowers. Trimming off all of the undergrowth (before starting flowering) will make this happen. Trust me, you will get plenty of pollen. ;)

Doing this reduces the possibility of mold and other problems. This also means, at harvest time, there's nothing to stop the fall of pollen except some branches.

  • Spray Every 5 Days
    All over the internet you'll see people recommending spraying a plant and tossing it in flower and only a few say to spray it again after 2 weeks. The rest of the horticultural world says STS is effective for about 5 days in a plant. The rest of the horticultural world sprays every 5-7 days.

I spray every 5 days. Starting 5 days before changing the lighting to a flowering schedule, spray the nodes of the plant every 5 days until 10% of the flower pods are open and dumping pollen. (Once the last spray is dry, lay out your collection trays, parchment paper or foil)

  • Pods Which Won't Open Can Still Be Viable
    Spraying every 5 days, the pods should open well and dump lots of pollen. Should you find a plant which grows pods and seems to quit, right before opening, you can still harvest the pollen. Clip the pods off and dry them in a low humidity area or dessicant chamber.

Over a piece of glass or parchment paper, rub the pods across a stainless steel mesh, like this small fine-mesh kitchen strainer. Pollen will drop through the screen, along with small bits of pod/plant matter. You'll also end up with plant matter which will not go through the screen. Rub until you're sure you've rubbed the majority of the pollen through the screen. A small brush comes in handy to clean the screen of pollen.

  • Dilute Your Pollen With Toasted Flour
    When pollen is mixed with toasted (and cooled) flour, it greatly reduces waste and helps contain the pollen when using it. Using straight pollen is wasteful and adding flour greatly increases your chances of spreading the pollen thin enough for full use. Adding flour also helps keep pollen from floating around the grow area, pulling it to the floor.

Toast 50 to 100 times as much flour as you have pollen in a dry pan over medium heat. Let it cool. Thoroughly mix your pollen into the cooled flour. I like using a stainless steel pan, becuse pollen and flour slide effortlessly across the bottom. Once it's completely mixed you can pollinate your flowers. (Watch my blog for a tutorial on how to collect and store pollen for use years later. Coming up in the next few weeks)

I Believe The Time I Spent on This Tutorial Was Worth It

I found some information I hadn't seen earlier and cemented some information a bit deeper for me. I'm also confident the tutorial can be followed easily and with great success.

I look forward to seeing lots of comments with photos of reversed plants. :D

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Here's a Link to a Donation Page for My Efforts Feel free to share a representation of how much this post helped you. Remember to come back next year with your updates. :D

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This post is under valued...

I must go to sleep now. So i didn't read it properly. But this materie-info is super interesting and imo controversial:) I resteem it so maybe later i get back on this topic.

Peace

Thank you

I believe with continued efforts to build awareness, this information will be valued by the masses. On a side note, the first seed (I've noticed) dropped off the plant today. My first feminized seed! I'm rather excited. lol

COME BACK ON STEEMIT! @canna-curate needs you!

Here's an Early Dane S1

From the seeds formed due to this tutorial. ;)Early-Dane-S1.png
Happy frog and plain tap water from the area. "Just" repotted about 10 minutes earlier. :D

Man- thats really fucking cool that you shared even how to make the solution!!

And even cooler to see a plant from one of the seeds!!

Thanks :)

I took the time to figure out how to do it right. It was so awesome I had to share. Glad you like it. :) Really amazing to see a super happy plant, from seeds you made yourself. Even more amazing to know there's only a tiny chance it will be male. lol

If you come through Cahone, Colorado I'll give you a couple. :)

Did you offer to sell them for steem or sbd on here?! im sure youll get some interest for that!

No I haven't, because it's illegal for me to ship them, and it's tax evasion if I accept money for them. I'm working on publishing a feminizing guide for cannabis, to sell alongside my other book. :)

Why? So I can spend another 2 weeks on a $1.66 article??

Learn how to use bidbots to promote your work (unless obviously you see them as bad!) and come join us @canna-curate to get around 40 upvotes on each post from the curation trail :)

Seriously dude, Steemit is still new and germinating, not even yet at the growing stage, but its getting there! Some cannabis lovers on here set up canna'curate as theres not much on the canna scene on here! So we are it!

This is true.

I find the cannabis community to be too small right now. I'm focused on other platforms and will still post content here, just not focused on steemit anymore.

When there are more cannabis users with education, I'll spend more time here. Probably in the next 2 years for sure. Most of what I post is really beyond the main crowd and they have no idea what I'm talking about. lol

Are you on discord? Im not one who is online 24\7 so one website is plenty for me lol. Steemit is good because of the currency Steem, what a great crypto!!

Not really. I open it occasionally to check on Axie Infinity. I'm working on promoting my book, so being on more than one platform helps. :) Enter for a chance to win a free copy by liking/following http://facebook.com/douglasshermancurtis if you're on facebook. :)

FYI The STS in the Covered Spray Bottle is Still Clear

It looks as if the solution I mixed half a year ago is still good. The silver looks non-oxidized, so I'm using it for another plant. There's enough left for a small plant.

The First Seed Has Dropped!

The Early Dane x Early Dane Feminized seeds have begun to separate from the plant!! Oh yeah! :D

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Awesome! Thank You! :D

Yes, it's my original material. ;) Thanks for checking!

Oh I realized it was original lol. If you tag them in a comment they upvote the post. If you look you got an upvote from them with around .01 I noticed you didn't do it so I did. I also got you a minnowsupportproject upvote

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The staying safe section is gold people sometimes forget to prorect themselves when getting into chemicals. Great post as expected 🤘

Thank You

I was worried for a while I'd never finish it. :) Still lots to do around here, so I did a "post editing marathon" the last 2 days. lol I was very surprised to have a cold hand for days, from a non-toxic substance, so yeah... very glad I'm able to share that tidbit of info. ;)

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Thank you, glad you approve. :D

Such a great post, great photos, and very educative information. 10-10-10 in any way you look, I truly wish you could get more for this post as feedback from more than 1400 people who already read this post. Looks like not very fair to me. Thank you very much for work you do and share with others.

Thank You!

Life is all about how you react to life. I'm happy 1400 folks have seen it! I appreciate the kind words, may your rewards be great! :)

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